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Image Search Results
Journal: Frontiers in Immunology
Article Title: An Attempt to Polarize Human Neutrophils Toward N1 and N2 Phenotypes in vitro
doi: 10.3389/fimmu.2020.00532
Figure Lengend Snippet: Enhanced survival of Leishmania donovani promastigotes in N2-polarized neutrophils in vitro . Primary human neutrophils were infected with L. donovani promastigotes (ratio 1:10) for 3 h at 37°C, 5% carbon dioxide (CO 2 ). Successful infection was determined by Giemsa staining of cytocentrifuged samples, representative microscopy shown in (A) . Arrows show representative ingested (intracellular) Leishmania . After removing the free, non-ingested parasites, the infected cells were incubated in the presence of an N1 or N2 polarization cocktail at 37°C, 5% CO 2 . Survival of parasites was assessed after 24 and 48 h by the limiting dilution assay (B) . Bar diagrams show mean ± SD ( n = 4). * p < 0.05.
Article Snippet: The preparations contained ≥99% granulocytes , of which >95% were neutrophils and 1%–4% were eosinophils, as determined by
Techniques: In Vitro, Infection, Staining, Microscopy, Incubation, Limiting Dilution Assay